SHENTEK utilizes magnetic separation techniques for isolating and purifying target proteins and peptides, in conjunction with 2D electrophoresis or LC-MS for assessing antibody coverage.
The process begins by coupling polyclonal antibodies to magnetic beads to specifically isolate or purify antigens of interest：
Antibody Immobilization: The antibody is covalently coupled to the magnetic beads, ensuring stable attachment to the bead surface.
Protein Binding: The HCP sample is exposed to the antibody-coated beads, allowing selective binding between the immobilized antibodies and the recognizable HCPs.
Washing: Unbound HCPs and contaminants are removed by washing the beads with appropriate buffers, ensuring high specificity and purity of the captured target.
Elution: The target HCPs are eluted from the beads under low pH conditions that disrupt the antibody-antigen interaction, releasing the purified target HCPs.
This approach retains the advantages of Antibody Affinity Extraction (AAE) chromatography, while the suspension-based mixing of immunomagnetic beads with HCP samples enhances binding efficiency. By employing magnetic beads, the method minimizes non-specific adsorption and hands-on time, improving consistent protein capture with high reproducibility.
HCP Antibody coverage analysis: IMBS will capture the HCP through the coupled HCP antibodies, and then the HCP analysis by 2D electrophoresis and LC-MS (Q-Exactive ultra-high-resolution mass spectrometry) analysis will provide customers with orthogonal coverage analysis results.
Custom Immunoaffinity Purification: The optimized binding using IMBS technology ensures specific interactions between the antibody and the target analyte, while effectively removing non-specific bindings. This enables a more precise and objective analysis for customers.
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