SHENTEK® Residual Sf9&AcNPV DNA Quantitation Kit is used to quantitate residual Sf9&AcNPV host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes multiplex quantitative PCR (qPCR) technique to perform a rapid, specific, and reliable quantitation assay at the femtogram (fg) level. IPC (Internal Positive Control) is included in the assay to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
| Components | Shipping Condition | Unit Size | Detection Method |
| Sf9&AcNPV DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| qPCR Reaction Buffer | -20℃, protect from light | ||
| Sf9&AcNPV Primer&Probe MIX | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | Sf9: 3x10-3-3x102 pg/μL, R2=0.99950，E=96.5% |
| AcNPV: 5.14x101 - 5.14x106 copies/μL, R2=0.99952，E=93.0% | |
| Accuracy | Sf9: Recovery = 80.0%-129.0%, CV<30% |
| AcNPV: Recovery = 80.4%-85.9%, CV<30% | |
| LOQ | Sf9: 0.0015 pg/μL |
| AcNPV: 51.4 copies/μL | |
| LOD | Sf9: 0.0003 pg/μL |
| AcNPV: 1.71 copies/μL | |
| Precision | Sf9: 6.0%-9.8% (<30%) |
| AcNPV: 4.1%-6.8% (<30%) | |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
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