Residual E.coli RNA Quantitation Kit (2G)

Name: Residual E.coli RNA Quantitation Kit (2G)
Brand: SHENTEKBIO

Product Number: 1201201-1

SHENTEK^® Residual E. coli RNA Quantitation Kit (2G) is used for quantitation of host cell RNA from E. coli in a variety of biopharmaceutical products. This kit employs duplex reverse transcription quantitative PCR technique to performs a rapid, specific, and reliable quantitative detection of residual E. coli RNA. The target gene (FAM) can be determined at the femtogram (fg) level, whereas IPC-Internal Positive Control (VIC) is also included in the E. coli RNA Primer&Probe MIX to evaluate the performance of each PCR reaction.

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Overview - SHENTEK^® Residual E.coli RNA Quantitation Kit (2G)

Components	Shipping Condition	Unit Size	Detection Method
E.coli RNA Control	-20℃	100 Reactions	Probe-based qPCR
One Step qPCR Buffer	-20℃, protect from light
One Step Enzyme MIX	-20℃, protect from light
E.coli RNA Primer&Probe MIX (Incl IPC)	-20℃, protect from light
RNase-Free H2O	-20℃

Specification - SHENTEK^® Residual E.coli RNA Quantitation Kit (2G)

Range	2x10^-3-2x10²pg/μL, R² =1.000，E=104.6%
Accuracy	64.1%-97.8%, CV<30%
LOQ	0.002 pg/μL
Precision	7.4%-11.0% (<30%)
Robustness	Freeze-thaw stability over 5 cycles
Robustness	Instrument suitability not limited to Thermo, Biorad, Bioer, SHENTEK qPCR equipment