SHENTEK® Replication-Competent Lentivirus (RCL) Quantitation Kit is used for the quantitative detection of replicable lentiviral RCL in cell therapy products and gene therapy products produced with lentiviral vectors, such as virus-producing cell banks, end of production cells, viral vectors and CAR-T cells. This kit is rapid, specific and reliable, and can work in coordination with the SHENTEK® Virus DNA & RNA Extraction Kit to quantitate the copy number of replicable lentivirus RCL in samples.
| Components | Shipping Condition | Unit Size | Detection Method |
| RCL Control | -20℃ | 100 Reactions | Probe-based qPCR |
| 5×RT-qPCR Buffer | |||
| RNase-Free H2O | |||
| ddH2O | |||
| RCL Primer&Probe MIX | -20℃, protect from light | ||
| RT-qPCR Enzyme MIX |
| Range | 6 - 6×105 copies/μL, R2= 0.99990，E=98.5% |
| Accuracy | Recovery = 90.0%-122.5%, CV<20% |
| LOQ | 6 copies/μL |
| LOD | 1 copies/μL |
| Precision | 2.9%-4.6% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, Roche, SHENTEK qPCR equipments |
SHENTEK® Virus DNA & RNA Extraction Kit
SHENTEK® Replication-Competent Retrovirus (RCR) Quantitation Kit
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