This kit is used to quantitate residual NS0&SP2/0 host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products.
The SHENTEK® Residual NS0&SP2/0 DNA Quantitation Kit utilizes quantitative PCR (qPCR) technique to perform a rapid, specific, and reliable quantitation assay at the femtogram (fg) level. The kit provides NS0&SP2/0 DNA Control as reference standard. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
| Components | Shipping Condition | Unit Size | Detection Method |
| NS0&SP2/0 DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| qPCR Reaction Buffer | -20℃, protect from light | ||
| NS0&SP2/0 Primer&Probe MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | 3 fg/μL~300 pg/μL, R2=0.999, E=93.4% |
| Accuracy | Recovery = 75.8%-107.7% , CV<15% |
| LOQ | 3 fg/μL |
| Precision | CV<15% |
| Robustness | Freeze-thaw stability over 4 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
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