Sf9 HCP ELISA Kit

This kit is based on the solid-phase enzyme-linked immunosorbent assay (ELISA) with a double-antibody sandwich technique to detect residual host cell proteins (HCPs) from Sf9 cells. A sheep polyclonal antibody specific to Sf9 HCPs was employed in the assay to capture any remaining HCPs in the sample. The antibody coverage is assessed by the current mainstream method. Both the Calibration standards (or test sample) and the HRP (Horseradish Peroxidase) labeled with anti-Sf9 HCP antibody were simultaneously added to the microtiter plate coated with the affinity purified capture antibody, and followed by incubation and washing. Then TMB (3,3',5,5' -tetramethylbenzidine) substrate was added into reaction, HRP catalyzed the oxidation of TMB by H₂O₂ to produce a blue colored product (maximum absorption peak at 655 nm). Then the stop solution was added to terminate the enzymatic reaction, resulting in a yellow colored product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength was positively correlated with the HCPs concentration in the calibration standard and the samples. The concentration of HCPs in the samples can be calculated using a dose-response curve.