SHENTEK® Residual MDCK DNA Quantitation Kit is used to quantitate residual MDCK host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes quantitative PCR (qPCR) technique to a perform rapid, specific, and reliable quantitation assay at the femtogram (fg) level. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
| Components | Shipping Condition | Unit Size | Detection Method |
| MDCK DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| qPCR Reaction Buffer | -20℃, protect from light | ||
| MDCK Primer&Probe MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | 3x10-3-3x102 pg/μL, R2=0.999，E=98.2% |
| Accuracy | Recovery = 68.0%-74.0%, CV<30% |
| LOQ | 0.003 pg/μL |
| Precision | 6.6%-12.7% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
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