This kit is used to quantitate Sf9 DNA residues of different fragment sizes in different stages of biopharmaceutical products, from in-process samples to final products.
The SHENTEK® Residual Sf9 DNA Size Analysis Kit is designed to amplify four different fragments (87bp, 118bp, 225bp and 500bp) for the accurate determination of their size distribution at the femtograms (fg) level.
For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Size: 4 × 100 Reactions
| Components | Shipping Condition | Unit Size | Detection Method |
| Sf9 DNA Control | -20℃ | 4×100 Reactions | Probe-based qPCR |
| qPCR Reaction Buffer | -20℃, protect from light | ||
| Sf9 Primer&Probe MIX-87 | |||
| Sf9 Primer&Probe MIX-118 | |||
| Sf9 Primer&Probe MIX-225 | |||
| Sf9 Primer&Probe MIX-500 | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | 87bp: 0.003 pg/μL-300 pg/μL, R2=0.99938 |
| 118bp: 0.003 pg/μL-300 pg/μL, R2=0.99980 | |
| 225bp: 0.003 pg/μL-300 pg/μL, R2=0.99858 | |
| 500bp: 0.003 pg/μL-300 pg/μL, R2=0.99952 | |
| Accuracy | 87bp: Recovery = 70.1%-87.1% , CV<40% |
| 118bp:Recovery = 79.6%-84.5% , CV<40% | |
| 225bp: Recovery = 79.3%-103.3% , CV<40% | |
| 500bp: Recovery = 74.9%-87.9% , CV<40% | |
| LOQ | 0.003 pg/μL, CV<40% |
| LOD | 87 bp, 118 bp, 225 bp: 0.0003 pg/μL |
| 500bp: 0.001 pg/μL | |
| Precision | CV<40% |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
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