This kit is used to quantitate MDCK DNA residues of different fragment sizes in different stages of biopharmaceutical products, from in-process samples to final products.
The SHENTEK® Residual MDCK DNA Size Analysis Kit is designed to amplify four different fragments (84bp, 142bp, 204bp and 504bp) for the accurate determination of their size distribution at the femtograms (fg) level.
For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Size: 4 × 100 Reactions
| Components | Shipping Condition | Unit Size | Detection Method |
| MDCK DNA Control | -20℃ | 4×100 Reactions | Probe-based qPCR |
| qPCR Reaction Buffer | -20℃, protect from light | ||
| MDCK Primer&Probe MIX-84 | |||
| MDCK Primer&Probe MIX-142 | |||
| MDCK Primer&Probe MIX-204 | |||
| MDCK Primer&Probe MIX-504 | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | 84bp: 0.03 pg/μL-3000 pg/μL, R2=0.990 |
| 142bp: 0.003 pg/μL-300 pg/μL, R2=0.990 | |
| 204bp: 0.003 pg/μL-3000 pg/μL, R2=1.000 | |
| 504bp: 0.003 pg/μL-3000 pg/μL, R2=0.990 | |
| LLOQ | 0.003 pg/μL, CV<20% |
| Precision | CV<40% |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
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