Medical Consumables and Lab Consumables OEM Manufacturer
Medical Consumables and Lab Consumables OEM Manufacturer

Mycobacteria DNA Detection Kit

Product Number: 1503602

MycoSHENTEK® Mycobacteria DNA Detection Kit is used, together with MycoSHENTEKTM Mycobacteria DNA Extraction Kit, to qualitatively determine whether there is Mycobacteria contamination in cells, cell culture-derived biologicals, etc., using real-time qPCR techonology. The integrated system has been fully validated at a detection limit of 10-100 CFU/mL to detect more than 100 different Mycobacteria species. The system contains dUTP, and if used with the UNG enzyme to prevent contamination and eliminate carry-over contamination Internal Control (IC) can be added to a PCR amplification reaction mixture to evaluate whether a test sample inhibits the amplification reaction and may result in false negative results. It can also be added to the sample before extraction to evaluate the extraction efficiency. For extraction information, please refer to MycoSHENTEK® Mycobacteria DNA Extraction Kit User Guide (Product No.: 1503601).

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Overview - MycoSHENTEK® Mycobacteria DNA Detection Kit

ComponentsShipping ConditionUnit SizeDetection Method
MB qPCR Reaction Buffer-20℃, protect from light50 ReactionsProbe-based qPCR
MB Primer & Probe MIX
MB Internal Control (IC)-20℃
MB Positive Control (PC)
DNA Dilution Buffer (DDB)






Specification - MycoSHENTEK® Mycobacteria DNA Detection Kit

InclusivityMore than 100 different Mycobacteria species
Detection Limit10 CFU/test or 20 copies/test (Positive cut-off: 95%)
SpecificityNo matrix-induced effect across different sample matrices and no cross-reactivity to different cell lines, bacteria or fungi
RobustnessFreeze-thaw stability over 5 cycles
Instrument suitability not limited to Thermo, Biorad, Roche, Jena, SHENTEK qPCR equipments
Consistent performance to the slight variations of reaction system and amplification procedure






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MycoSHENTEK® Mycobacteria DNA Extraction Kit

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