SHENTEK® Residual Pichia Pastoris DNA Quantitation Kit is used to quantitate residual Pichia Pastoris DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit utilizes fluorescent quantitative PCR technique (FAM) to perform rapid and specific quantitation of residual Pichia Pastoris DNA fragments in samples, and reliable quantitation assay at the fg level. Pichia Pastoris DNA Control is provided as reference standard. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
| Components | Shipping Condition | Unit Size | Detection Method |
| Pichia Pastoris DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| Pichia Pastoris qPCR Reaction Buffer | -20℃, protect from light | ||
| Pichia Pastoris Primer&Probe MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | 3x10-2-3x102 pg/μL, R2=0.99976，E=95.0% |
| Accuracy | Recovery = 78.8%~110.6%, CV < 30% |
| LOQ | 0.03 pg/μL |
| LOD | 0.001 pg/μL |
| Precision | 4.3%-8.8% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
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