SHENTEK® CAR/TCR Transgene Copy Number Quantitation Kit is designed to quantify the copy number of target genes in human cell products derived from common HIV-1 lentiviral vectors, such as CAR or TCR genes integrated in CAR-T and TCR-T cell genomes.
This kit utilizes multiplex qPCR technique to detect DNA sequences related to the integration or expression function on the transfer plasmid and Single Copy Gene (SCG) in human cells. Average copies of target gene per cell can be calculated in the samples, such as CAR or TCR gene copy numbers. This kit is rapid, specific, reliable, and universal for lentiviral vector applications. The reference standard in this kit is produced by our company.
| Components | Shipping Condition | Unit Size | Detection Method |
| qPCR Reaction Buffer | -20℃, protect from light | 100 Reactions | Probe-based qPCR |
| CAR/TCR Primer&Probe MIX | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ | ||
| CAR/TCR Control |
| Range | CAR/TCR: 2.33×101-2.33×107 copies/μL, R2= 0.999829，E=94.9% |
| SCG: 2.33×102-2.33×107, R2= 0.999564, E=94.8% | |
| Accuracy | Recovery = 104.7%-137.6%, CV<20% |
| LOQ | CAR/TCR: 2.33×101 copies/μL |
| SCG: 5.285×101 copies/μL | |
| LOD | 2.33 copies/μL |
| Precision | 5.9%-9.1% (<15%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, Bioer, SHENTEK qPCR equipments |
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