SHENTEK® Residual SV40LTA & E1A DNA Quantitation Kit (2G) is used to quantitate residual SV40LTA & E1A host cell DNA, such as HEK293T cell. This kit uses duplex real-time PCR technique to performs rapid, specific, and reliable quantitation assay at the level of 101 copies/μL. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191)
| Components | Shipping Condition | Unit Size | Detection Method |
| SV40LTA & E1A linear DNA Control | -20℃ | 100 Reactions | Probe-based qPCR |
| SV40LTA & E1A non-linear DNA Control | |||
| qPCR Reaction Buffer | -20℃, protect from light | ||
| SV40LTA & E1A Primer & Probe MIX | |||
| IPC MIX | |||
| DNA Dilution Buffer (DDB) | -20℃ |
| Range | SV40LTA: 4.67x101-4.67x106 pg/μL, R2=0.99974, E=97.5% |
| E1A: 4.97x101-4.97x106 pg/μL, R2=0.99946, E=98.3% | |
| Accuracy | SV40LTA: Recovery = 105.2%-110.1%, CV<30% |
| E1A: Recovery = 115.8%-124.5%, CV<30% | |
| LOQ | SV40LTA: 9.34 copies/μL |
| E1A: 49.7 copies/μL | |
| LOD | SV40LTA: 0.934 copies/μL |
| E1A: 0.497 copies/μL | |
| Precision | SV40LTA: 1.7%-9.0% （<30%） |
| E1A: 1.9%-9.4%（<30%） | |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments |
info@shentekbio.com 
English