CHO K1 HCP ELISA Kit (One-step ELISA)

The kit employs a solid-phase Enzyme-linked Immunosorbent Assay (ELISA) with a double-antibody sandwich technique to detect residual CHO K1 host cell proteins in the samples. Polyclonal antibody specific to CHO K1 HCPs was employed in the assay to capture any remaining HCPs in the samples. Both the Calibration standards (or test samples) and the HRP (Horseradish Peroxidase) labeled anti-CHO K1 HCPs antibody were simultaneously added to the microtiter plate, which coated with the affinity purified capture antibody and followed by incubation and washing. Then TMB (3,3',5,5' -tetramethylbenzidine) substrate was added into the reaction, HRP catalyzed the oxidation of TMB by H₂O₂ to produce a blue product (maximum absorption peak at 655 nm). The stop solution is added to terminate the enzymatic reaction, resulting in a yellow color product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength were positively correlated with the HCPs concentration in the Calibration standards and the samples. The concentration of CHO K1 HCPs in the samples can be calculated using the dose-response curve.