SHENTEK® Virus DNA & RNA Extraction Kit is suitable for extraction of viral DNA and RNA from up to 107 cells, and also for nucleic acid extraction of harvested virus bulk (liquid). The kit is compatible with either manual sample preparation or automated extraction with SHENTEK® rHCDpurify system. Depending on subcellular location of viral replication, the extraction methods for cells can be grouped into two categories:
Method 1: For viruses replicated in host nucleus including retroviruses, such as most DNA viruses (rcAAV/rAAV) as well as replication competent lentivirus (RCL) and relication competent retrovirus (RCR), additional nuclease digestion after cell lysis can reduce the risk of false negative. Method 1 is also recommended for detection of samples contaminated with unknown viruses.
Method 2: For viruses replicated in host cytoplasm, for example most RNA viruses, cell lysis followed by centrifugation and extraction of the supernatant is sufficient for detection purposes.
| Components | Shipping Condition | Unit Size | Detection Method |
| Wash buffer A | room temperature | 50 Extractions | Magnetic particle separation |
| Binding solution | |||
| Proteinase K Buffer | |||
| Pretreatment buffer | 2-8℃ | ||
| Magnetic particles | |||
| 5M NaCl | |||
| Precipitation solution I | -20℃ | ||
| RNase-Free H2O | |||
| Proteinase K |
| Recovery | at least 90% to fg/μL |
| Repeatability | CV no more than 15% |
SHENTEK® Replication-Competent Lentivirus (RCL) Quantitation Kit
SHENTEK® Replication-Competent Retrovirus (RCR) Quantitation Kit
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