SHENTEK® Residual E. coli DNA Quantitation Kit is used to quantitate residual E. coli host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to perform rapid, specific, and reliable quantitation assay at the fg level. IPC (Internal Positive Control) is included in the E. coli Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
| Components | Shipping Condition | Unit Size | Product Line | Detection Method |
| E. coli DNA Control | -20℃ | 100 Reactions | SHENTEK® | Probe-based qPCR |
| qPCR Master MIX | ||||
| DNA Dilution Buffer (DDB) | ||||
| E. coli Primer&Probe MIX (Incl IPC) | -20℃, protect from light |
| Range | 3x10-2-3x102 pg/μL, R2=0.99968, E=97.5% | ||
| Accuracy | Recovery = 81.2%-107.4% , CV≤30% | ||
| LOQ | 0.03 pg/μL | ||
| LOD | 0.003 pg/μL | ||
| Precision | 2.8%-17.9% (CV≤30%) | ||
| Specificity | No cross-reactivity with CHO, 293T and P.pastoris cells. | ||
| Robustness | Freeze-thaw stability over 5 cycles | ||
| Instrument suitability not limited to Thermo, Biorad, SHENTEK qPCR equipments | |||
SHENTEK® Residual Host Cell DNA Sample Preparation Kit
SHENTEK® Residual E. coli DNA Size Analysis Kit (2G)
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SHENTEK® E.coli (Protein Expression Strains) HCP ELISA Kit (One-step ELISA)
SHENTEK® E.coli (K-12 & Alkaline Lysis) HCP ELISA Kit
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