Lentivirus vector are used to introduce CAR or TCR genetic sequence into cell therapy products. However, due to the variations among different individuals' genomes, there maybe variable copy numbers of the lentivirus-specific TCR gene transferred into peripheral T cells, resulting in different cyto-toxicity in heterogeneous CAR-T cell populations.
SHENTEK CAR/TCR Gene Copy Number Quantification Kit is are liable and accurate quality control test for the detection of transgene copy number in CAR-T cells that are transfected with rHIV-1 lentiviral vectors.
Range: CAR/TCR: 2.33×101-2.33×107 copies/μL, R2= 0.999829,E=94.9%
SCG: 2.33×102-2.33×107, R2= 0.999564,E=94.8%
LOQ: CAR/TCR: 2.33×101 copies/μL
SCG: 5.285×101 copies/μL
LOD: 2.33 copies/μL
Precision: 5.9%-9.1% (CV<15%)